Saline:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
Incubation:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
Blocking Assay:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
Activation Assay:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
Western Blot:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
Expressing:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
Control:Article Title: Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Article Snippet: The membranes were blocked with blocking buffer (TBS, 0.1% Tween-20, 5% non-fat milk or 2% BSA) for 2 h at room temperature and incubated with primary antibodies against NOX4 (ABclonal, Wuhan, China, Cat# A11274, 1:1000 dilution), HO-1 (Proteintech, Wuhan, China, Cat# 10701-1-AP, 1:1000 dilution), NQO1 (Proteintech, Wuhan, China, Cat# 67240-1-Ig, 1:1000 dilution), MFN1 (ABclonal, Wuhan, China, Cat# A9880, 1:1000 dilution), MFN2 (ABclonal, Wuhan, China, Cat# A19678, 1:1000 dilution), FIS1 (ABclonal, Wuhan, China, Cat# A19666 1:1000 dilution), TGF-β1 (Proteintech, Wuhan, China, Cat# 21898-1-AP, 1:1000 dilution), α-SMA (Proteintech, Wuhan, China, Cat# 14395-1-AP, 1:5000 dilution), collagen 1 (Proteintech, Wuhan, China, Cat# 67288-1-Ig, 1:5000 dilution), and GAPDH (ABclonal, Wuhan, China, Cat# AC001, 1:10,000 dilution) at 4 °C overnight, respectively.
Article Title: Naked mole-rat brown fat thermogenesis is diminished during hypoxia through a rapid decrease in UCP1
Article Snippet: Levels of specific proteins were determined using the following primary antibodies: UCP1 antibody (1:3000; Sigma Aldrich, #U6382), total OXPHOS rodent cocktail (1:2000; Abcam, #Ab110413), anti-ubiquitin (1:2000; Abcam, #Ab7780), LC3B (1:1000; Cell Signaling Technology, #2775), PARKIN (1:2000; ABclonal, #A0968), p62 (1:1000; ABclonal, #A11483), PINK1 (1:2000; ABclonal, #A7131), FUNDC1 (1:2000; ABclonal, #16318), BNIP3 (1:1000; Abcam, #Ab10433), BNIP3L (NIX; 1:250; Santa Cruz, #SC-166314), UCP3 (1:1000; Abcam, #AB3477), Fis1 (1:1000; BioVision, #3491), Drp1 (1:1000; BD Biosciences, #611118), Drp1-S616 (1:1000; ABclonal, #AP0849), Drp1-S637 (1:1000; Cell Signaling Technology, #4867), Casp3 (1:1000; ABclonal, #A0214), p53 (1:200; Cell Signaling Technology, #2524), AIF (1:2000; Abcam, #ab32516), BAX (1:500; Santa Cruz, #SC-526), Bcl2 (1:1000; Santa Cruz, #sc-492), Mff (1:1000; ABclonal, #A4874), Opa1 (1:2000; ABclonal, #A9833), Mfn1 (1:2000; ABclonal, #A9880), Mfn2 (1:1000; ABclonal, #A19678), TOM20 (1:2000; ABclonal, #A19403), TBC1D15 (1:2000; ABclonal, #A10593), RAB7A (1:2000; ABclonal, #A12784), and anti-ubiquitin antibody (1:2000; Abcam, #Ab7780).
Article Title: Decreased Mrpl42 expression exacerbates myocardial ischemia and reperfusion injury by inhibiting mitochondrial translation.
Article Snippet: Primary antibodies against the following proteins were used: β-actin (ABclonal Technology Co., Ltd., China, AC004), HSP90 (Cell Signaling Technology, #4874S), Mrpl42 (ABclonal Technology Co., Ltd., China, A12811), cleavedcaspase 3 (Cell Signaling Technology, #9664), MT-ND1 (ABclonal Technology Co., Ltd., China, A18316), MT-ND2 (ABclonal Technology Co., Ltd., China, A17968), MT-ND3 (ABclonal Technology Co., Ltd., China, A17969), MT-ND4 (ABclonal Technology Co., Ltd., China, A9941), MT-ND5 (ABclonal Technology Co., Ltd., China, A17972), MTND6 (ABclonal Technology Co., Ltd., China, A17991), MT-COX1 (ABclonal Technology Co., Ltd., China, A17889), MT-COX2 (ABclonal Technology Co., Ltd., China, A17965), MT-COX3 (ABclonal Technology Co., Ltd., China, A17891), MT-ATP6 (ABclonal Technology Co., Ltd., China, A17960), MT-ATP8 (ABclonal Technology Co., Ltd., China, A17890), VDAC1 (ABclonal Technology Co., Ltd., China, A19707), Nrf2 (ABclonal Technology Co., Ltd., China, A1244), puromycin (ABclonal Technology Co., Ltd., China, A21205), Drp1 (ABclonal Technology Co., Ltd., China, A21968), Mfn1 (ABclonal Technology Co., Ltd., China, A21293), Mfn2 (ABclonal Technology Co., Ltd., China, A19678), Opa1 (ABclonal Technology Co., Ltd., China, A9833), p-Drp1 (S616) (Cell Signaling Technology, Inc. #3455) and p-Drp1 (S637) (Abcam Limited.
Article Title: The role of TRAP1 in regulating mitochondrial dynamics during acute hypoxia-induced brain injury.
Article Snippet: The membranes were blocked with 5% skim milk prepared in tris buffered saline with Tween (TBST) for 2 h at room temperature and incubated with primary antibodies (TRAP1: 1:1000, Cell Signaling Technology; DRP1: 1:800, Cell Signaling Technology; MFN1: 1:1000, Abclonal; MFN2: 1:1000, Abclonal; FIS1: 1:800, Ablonal; β-actin: 1:500, Abcam) in antibody diluent solution (0.5% BSA) overnight at 4 °C.
Article Title: ATPAF1 deficiency impairs ATP synthase assembly and mitochondrial respiration
Article Snippet: LC3 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN1 (1:1000, ABclonal Biotechnology, Wuhan, China), MFN2 (1:1000, ABclonal Biotechnology, Wuhan, China), OPA1 (1:1000, ABclonal Biotechnology, Wuhan, China), DRP1 (1:1000, ABclonal Biotechnology, Wuhan, China), CI-NDUFB8 (1:1000, ABclonal Biotechnology, China) CII-SDHB (1:1000, ABclonal Biotechnology, China), CIII-UQCRC2 (1:1000, ABclonal Biotechnology, China), CIV-MTCO1 (1:1000, ABclonal Biotechnology, China); Horseradish peroxidase (HRP) linked secondary antibodies anti-mouse IgG (1:5000) and anti-rabbit IgG (1:5000) were from Boster Biological Tech, Wuhan, China.
|
ABclonal Biotechnology is a verified supplier 